In contrast to target-based drug discovery, phenotypic screening discloses the effect of a compound on a biological system without revealing its mode of action or its interaction sites. Phenotypic screening has delivered several new valuable drugs, however, the major challenge remains target deconvolution (1). In recent years, “omics” techniques and bioinformatics have brought significant advances to target identification in drug discovery phenotypic screening. However, such analyses can become ...
Ref.-Nr.: 1200-3727 BC
BiQ Analyzer is a software tool for easy visualization and quality control of DNA methylation data. With more than a thousand users worldwide, BiQ Analyzer has become a standard tool for processing DNA methylation data from bisulfite sequencing. BiQ Analyzer has been selected by ABI to be part of the Applied Biosystems Software Community Program.
Ref.-Nr.: 0903-5658-LC; 0903-5696-LC; 0903-5692-LC
Bildgebung und Mikroskopie
Super-resolution microscopy critically relies on the availability of appropriate fluorophores. For single-molecule localization microscopy (SMLM), fluorophores are needed that stochastically switch between bright (on) and dark (off) states. This phenomenon is also called fluorescence blinking. For stimulated emission depletion microscopy (STED) the dyes must be photo-switchable.
Prozesse und Methoden (inkl. Screening) : Lebens-Wissenschaften (inkl. Screening)
Medizin : Diagnostika
Nukleinsäure-, Protein-, Zell-bezogene Technologien : Protein (bezogen)
Extremely sensitive, reliable and fast novel KDAC assay, which can be performed in a continuous format. This novel KDAC assay is based on Firefly luciferase harboring an acetylation on an essential active site lysine, incorporated by genetic code expansion. We furthermore offer a bacterial selection system for KDACs, which uses a reporter enzyme inactivated by lysine modifications at an essential active site residue, thereby linking deacetylase activity to a selectable output.
Medizin : Therapeutika
Medizin : Diagnostika
Forschungs-Reagenzien : Andere
Nukleinsäure-, Protein-, Zell-bezogene Technologien : Zellen (bezogen)
Biomarkers of ageing have long been sought after to help assess the biological age and general health status of individuals. Considerable efforts have been invested to identify such biomarkers, including physiologic readouts, metabolic parameters, glycomic profiles and others. Nevertheless, markers with strong predictive power have remained elusive.
Work in model organisms has identified several conserved signaling pathways that extend life span, such as reduced insulin/IGF signaling and ...
Novel method for analysis of complex carbohydrate structures by ion-mobility mass spectrometry (IM-MS)
Carbohydrates form one of the major classes of biological macromolecules in living organisms, but structural analysis and the quality control of carbohydrate structures represent a major challenge due to the frequent occurrence of isomers that are difficult to distinguish using established techniques.
Changes in protein glycosylation are frequently identified in the context of diseases. For example, alterations in N-glycan profiles from blood plasma have been found to correlate with pathological ...
Prozesse und Methoden (inkl. Screening) : Chemische
Scientists in the group of Atmospheric Chemistry at the Max Planck Institute for Chemistry in Mainz have developed a novel thiosulfate based ozone scrubber to ensure accurate measurements of trace analytes. It is known from the literature that commercial potassium iodide (KI) containing ozone scrubbers can cause errors in the measurement of carbonyl compounds such as aldehydes and ketones due to byproducts formed from the oxidation of KI. Commercial ozone scrubbers based on sodium thiosulfate ...
A simple, one-step assay for reliable measurements of NAD(H) and NADP(H) in complex samples, with various applications such as enzymatic assays, clinicals tests, and as point of care tests in the field of ageing.